Examinando por Autor "Aguirre, Jacobo"

Mostrando 1 - 4 de 4

Acceso Abierto
A Multiplex Immunosensor for Detecting Perchlorate-Reducing Bacteria for Environmental Monitoring and Planetary Exploration
(Extreme Microbiology, 2020-12-16) Gallardo Carreño, Ignacio; Moreno Paz, M.; Aguirre, Jacobo; Blanco, Yolanda; Alonso Pintado, Eduardo; Raymond Bouchard, Isabelle; Maggiori, Catherine; Rivas, Luis A.; Engelbrektson, Anna; Whyte, Lyle; Parro García, V.; Ministerio de Economía y Competitividad (MINECO); Agencia Estatal de Investigación (AEI); Ministerio de Ciencia e Innovación (MICINN); Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737; Centros de Excelencia Severo Ochoa, CENTRO NACIONAL DE BIOTECNOLOGIA (CNB), SEV-2017-0712
Perchlorate anions are produced by chemical industries and are important contaminants in certain natural ecosystems. Perchlorate also occurs in some natural and uncontaminated environments such as the Atacama Desert, the high Arctic or the Antarctic Dry Valleys, and is especially abundant on the surface of Mars. As some bacterial strains are capable of using perchlorate as an electron acceptor under anaerobic conditions, their detection is relevant for environmental monitoring on Earth as well as for the search for life on Mars. We have developed an antibody microarray with 20 polyclonal antibodies to detect perchlorate-reducing bacteria (PRB) strains and two crucial and highly conserved enzymes involved in perchlorate respiration: perchlorate reductase and chlorite dismutase. We determined the cross-reactivity, the working concentration, and the limit of detection of each antibody individually and in a multiplex format by Fluorescent Sandwich Microarray Immunoassay. Although most of them exhibited relatively high sensitivity and specificity, we applied a deconvolution method based on graph theory to discriminate between specific signals and cross-reactions from related microorganisms. We validated the system by analyzing multiple bacterial isolates, crude extracts from contaminated reactors and salt-rich natural samples from the high Arctic. The PRB detecting chip (PRBCHIP) allowed us to detect and classify environmental isolates as well as to detect similar strains by using crude extracts obtained from 0.5 g even from soils with low organic-matter levels (<103 cells/g of soil). Our results demonstrated that PRBCHIP is a valuable tool for sensitive and reliable detection of perchlorate-reducing bacteria for research purposes, environmental monitoring and planetary exploration.
Acceso Abierto
Emergence of complex socioeconomic networks driven by individual and collective interests
(APS Physics, 2020-12-09) Iranzo, Jaime; Martí, Federico Pablo; Aguirre, Jacobo; Agencia Estatal de Investigacion (AEI); 0000-0003-2196-5103; [0000-0002-0633-6985; Centros de Excelencia Severo Ochoa, CENTRO NACIONAL DE BIOTECNOLOGIA (CNB), SEV-2017-0712; Centros de Excelencia Severo Ochoa, CENTRO DE BIOTECNOLOGIA Y GENOMICA DE PLANTAS (CBGP), SEV-2016-0672; Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
The basic mechanisms that lead to the creation of complex socioeconomic structures remain poorly understood. A combination of network science and game theory can help explain them by examining how simple networks interact to build complex entities, both when connections among individuals are exclusively guided by self-interest or when they result from a mixture of individual and collective motivations. Here we present a theoretical framework where individuals or human groups from different communities connect to each other only if they increase their own eigenvector centrality, a topological measure of wide applications in many different contexts that quantifies the importance of a node within the network. Our analytical and numerical results show that the emergence of interconnected networks is catalyzed by the self-interest of peripheral agents, who are penalized in the long run but transiently benefit from establishing links with nodes from other communities. Moreover, the interconnection process leads to a hierarchical, assortative, and very efficient structure where links across networks involve nodes of the same importance. These findings are robust to the introduction of moderate levels of collective-oriented behavior and compatible with the interconnection dynamics observed in real-world socioeconomic networks.
Restringido
Mars-like UV Flux and Ionizing Radiation Differently Affect Biomarker Detectability in the Desert Cyanobacterium Chroococcidiopsis as Revealed by the Life Detector Chip Antibody Microarray
(Mary Ann Liebert, Inc., publishers, 2022-10-03) Billi, Daniela; Blanco, Yolanda; Ianneo, Andrea; Moreno Paz, M.; Aguirre, Jacobo; Baqué, Mickael; Moeller, Ralf; De Vera, Jean Pierre; Parro García, V.
The effect of a Mars-like UV flux and γ-radiation on the detectability of biomarkers in dried cells of Chroococcidiopsis sp. CCMEE 029 was investigated using a fluorescence sandwich microarray immunoassay. The production of anti-Chroococcidiopsis antibodies allowed the immunoidentification of a reduced, though still detectable, signal in dried cells mixed with phyllosilicatic and sulfatic Mars regolith simulants after exposure to 6.8 × 105 kJ/m2 of a Mars-like UV flux. No signal was detected in dried cells that were not mixed with minerals after 1.4 × 105 kJ/m2. For γ-radiation (60Co), no detectable variations of the fluorescence signal occurred in dried cells exposed to 113 kGy compared to non-irradiated dried cells. Our results suggest that immunoassay-based techniques could be used to detect life tracers eventually present in the martian subsurface in freshly excavated materials only if shielded from solar UV. The high structural integrity of biomarkers irradiated with γ-radiation that mimics a dose accumulated in 13 Myr at 2 m depth from the martian surface has implications for the potential detectability of similar organic molecules/compounds by future life-detection missions such as the ExoMars Rosalind Franklin rover.
Restringido
The resumption of sports competitions after COVID-19 lockdown: The case of the Spanish football league
(Elsevier BV, 2020-06-04) Buldú, J. M.; Antequera, D. R.; Aguirre, Jacobo; Comunidad de Madrid; Agencia Estatal de Investigación (AEI); Budú, J. M. [0000-0002-9345-599X]; Jacobo, A. [0000-0003-2196-5103]; Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
In this work, we present a stochastic discrete-time SEIR Susceptible-Exposed-Infectious-Recoveredmodel adapted to describe the propagation of COVID-19 during a football tournament. Specifically, we are concerned about the re-start of the Spanish national football league, La Liga, which is currently –May 2020– stopped with 11 fixtures remaining. Our model includes two additional states of an individual, confined and quarantined, which are reached when an individual presents COVID-19 symptoms or has undergone a virus test with a positive result. The model also accounts for the interaction dynamics of players, considering three different sources of infection: the player social circle, the contact with his/her team colleagues during training sessions, and the interaction with rivals during a match. Our results highlight the influence of the days between matches, the frequency of virus tests and their sensitivity on the number of players infected at the end of the season. Following our findings, we finally propose a variety of strategies to minimise the probability that COVID-19 propagates in case the season of La Liga was re-started after the current lockdown.