Examinando por Autor "Laguna Castro, M."

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Acceso Abierto
Linking morphological and molecular sources to disentangle the case of Xylodon australis
(Nature Research Journals, 2020-12-15) Fernández-López, Javier; Telleria, Teresa M.; Dueñas, Margarita; Laguna Castro, M.; Schliep, Klaus; Martín, María P.; Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
The use of different sources of evidence has been recommended in order to conduct species delimitation analyses to solve taxonomic issues. In this study, we use a maximum likelihood framework to combine morphological and molecular traits to study the case of Xylodon australis (Hymenochaetales, Basidiomycota) using the locate.yeti function from the phytools R package. Xylodon australis has been considered a single species distributed across Australia, New Zealand and Patagonia. Multi-locus phylogenetic analyses were conducted to unmask the actual diversity under X. australis as well as the kinship relations respect their relatives. To assess the taxonomic position of each clade, locate.yeti function was used to locate in a molecular phylogeny the X. australis type material for which no molecular data was available using morphological continuous traits. Two different species were distinguished under the X. australis name, one from Australia–New Zealand and other from Patagonia. In addition, a close relationship with Xylodon lenis, a species from the South East of Asia, was confirmed for the Patagonian clade. We discuss the implications of our results for the biogeographical history of this genus and we evaluate the potential of this method to be used with historical collections for which molecular data is not available.
Acceso Abierto
Long-Range Cooperative Disassembly and Aging During Adenovirus Uncoating
(APS Physics (American Physical Society), 2021-04-30) Martín González, N.; Ibáñez Freire, P.; Ortega Esteban, Á.; Laguna Castro, M.; San Martín, C.; Valbuena, A.; Delgado Buscaloni, R.; De Pablo, P. J.; Agencia Estatal de Investigación (AEI); Ministerio de Economía y Competitividad (MINECO); Martín González, N. [0000-0001-6036-404X]; Ibáñez Freire, P. [0000-0001-6098-9912]; Ortega Esteban, A. [0000-0001-9642-0568]; Laguna Castro, M. [0000-0003-2237-1403]; San Martín, C. [0000-0001-9799-175X]; Valbuena, A. [0000-0002-6801-640X]; Delgado Buscalioni, R. [0000-0001-6637-2091]; De Pablo, P. J. [0000-0003-2386-3186]; Centros de Excelencia Severo Ochoa, CENTRO NACIONAL DE BIOTECNOLOGIA (CNB), SEV-2017-0712; Unidad de Excelencia Científica María de Maeztu CENTRO DE INVESTIGACIÓN DE FÍSICA DE LA MATERIA CONDENSADA (IFIMAC), MDM-2014-0377
Icosahedral virus capsids are closed shells built up with a hexagonal lattice of proteins, which incorporate pentamers at their fivefold vertices. Human adenovirus particles lose pentamers (pentons) during infection under a variety of physicochemical cues, including mechanical pulling of molecular motors and the viscous drag of the cytoplasm. By combining atomic force microscopy experiments with survival analysis and Markovian transition state theory, we investigate the sequence of adenovirus penton disassembly that reveals the aging of the virus structure. We show evidence that the lifetime of pentons gradually decreases, accompanied by capsid softening as neighboring pentons are lost. This cooperative dismantling process, which involves first-neighbor penton-penton distances of at least 45 nm, leads to a 50% increase in the virus disassembling rate of the virus particle. Theory and experiments fit remarkably well, allowing us to obtain the spontaneous escape rate and the energy barrier of penton disassembly ( ∼ 30 k B T ). The observed increase in the penton’s loss rate reveals long-range structural correlations within the capsid. Our estimations suggest that the mechanical cues arising from the strokes of protein motors carrying the virus to the nucleus could help penton disassembly and warrant the timely delivery of weak-enough capsids for adenovirus infection.