Examinando por Autor "Nazarious, Miracle Israel"

Mostrando 1 - 6 de 6

Acceso Abierto
ATMO-vent: An adapted breathing atmosphere for COVID-19 patients
(Elsevier BV, 2020-09-26) Mathanlal, Thasshwin; Nazarious, Miracle Israel; Mantas Nakhai, R.; Zorzano, María Paz; Luleå University of Technology (LTU); Mathanlal, T. [0000-0003-2691-3855]; Israel Nazoriuos, M. [0000-0002-7148-8803]; Mantas Nakhai, R. [0000-0002-4474-9983]; Zorzano, M. P. [0000-0002-4492-9650]; Martín Torres, J. [0000-0001-6479-2236]; Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
The ongoing worldwide pandemic of coronavirus disease 2019 (COVID-19), has been one of the most significant challenges to humankind in centuries. The extremely contagious nature of the SARS-CoV-2 virus has put forth an immense pressure on the health sector. In order to mitigate the stress on the healthcare systems especially to battle the crisis of mechanical ventilators, we have designed a modular, and robust DIY ventilator, ATMO-Vent (Atmospheric Mixture Optimization Ventilator) which can be fully mounted within two days by two operators. The ATMO-Vent has been designed using low-cost, robust, Commercial Off The Shelf (COTS) components, with many features comparable to a full-fledged ventilator. ATMO-Vent has been designed based on the United Kingdom Medicines & Healthcare products Regulatory Agency (UK-MHRA) guidelines for Rapidly Manufactured Ventilator System (RMVS), yet is scalable to the specific requirements of different countries. ATMO-Vent is capable of adjusting the Fraction of Inspiratory Oxygen (FiO2) levels, Tidal Volume (TV), frequency of breaths, Inspiratory/Expiratory ratio (I/E), Peak Inspiratory Pressure (PIP) and Positive End-Expiratory Pressure (PEEP). ATMO-Vent can operate in two modes – Continuous Mandatory Ventilation (CMV) using Volume-Controlled Ventilation (VCV) and in Assisted Control (AC) mode with pressure triggered by the patient. ATMO-Vent has undergone rigorous testing and qualifies under Class B Electric and Magnetic Compatibility (EMC) requirements of EN 55,011 CISPR 11 standards.
Acceso Abierto
Implementing bioburden reduction and control on the deliquescent hydrogel of the HABIT/ExoMars 2022 instrument.
(Elsevier BV, 2020-04-21) Mathanlal, Thasshwin; Nazarious, Miracle Israel; Vakkada Ramachandran, A.; Zorzano, María Paz; Martín Torres, Javier; Rettberg, P.; Rettberg, P. [0000-0003-4439-2395]; Zorzano, M. P. [0000-0002-4492-9650]; Martín Torres, J. [0000-0001-6479-2236]; Vakkada Ramachandran, A. [0000-0003-0499-6370]; Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
The HabitAbility: Brines, Irradiation and Temperature (HABIT) instrument will be part of the ExoMars 2022 mission (ESA/Roscosmos) and will be the first European In-situ Resource Utilization (ISRU) instrument capable of producing liquid water on Mars. HABIT is composed by two modules: Environmental Package (EnvPack) and Brine Observation Transition To Liquid Experiment (BOTTLE). EnvPack will help to study the current habitability conditions on Mars investigating the air and surface thermal ranges and Ultraviolet (UV) irradiance; and BOTTLE is a container with four independent vessels housing deliquescent salts, which are known to be present on Mars, where the liquid water will be produced after deliquescence. In order to prevent capillarity of deliquescent or hydrated salts, a mixture of deliquescent salts with Super Absorbent Polymer (SAP) based on polyacrylamide is utilized. This mixture has deliquescent and hydrogel properties and can be reused by applying a thermal cycle, complying thus with the purpose of the instrument. A High Efficiency Particulate Air (HEPA) grade filter made of polytetrafluroethylene (PTFE) porous membrane sandwiched between spunbounded nonwoven fabric stands as a physical barrier allowing interaction between the gaseous molecules of the Martian atmosphere and the salt mixtures, and at the same time preventing the passage of any potential biological contamination from the cells to the outside or vice-versa. In addition to the physical barrier, a strict bioburden reduction and analysis procedure is applied to the hardware and the contained salt mixtures adhering to the European Cooperation for Space Standardization protocol of microbial examination of flight hardware (ECSS-Q-ST-70-55C). The deliquescent salts and the SAP products need to be properly treated independently to adhere to the planetary protection protocols. In this manuscript, we describe the bioburden reduction process utilized to sterilize the salt mixtures in BOTTLE and the assays adopted to validate the sterilization. We also describe the construction of a low-cost, portable ISO 7 cleanroom tent, exclusively designed for planetary protection tests. The sterilization process involves Dry Heat Microbial Reduction (DHMR) of the deliquescent salts and the SAP mixtures. The performance of SAP after DHMR is validated to ensure its working efficiency after sterilization. A slightly modified version of the standard swab assay is used in the validation process and a comparison is made between samples exposed to a thermal shock treatment and those without thermal shock, to determine the best assay to be applied for future space hardware utilizing such salt mixtures for planetary investigation and In-Situ Resource Utilization (ISRU). The demonstration of the compatibility of these products with the processes commonly required for space applications has implications for the future exploration of Mars.
Restringido
Measuring electrical conductivity to study the formation of brines under martian conditions
(Cambridge University Press, 2021-07-28) Nazarious, Miracle Israel; Ramachandran, A. V.; Zorzano, María Paz; Martín Torres, Javier; Swedish National Space Agency (SNSA); Agencia Estatal de Investigación (AEI); Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
This paper describes a protocol to design experiments to study the formation of brines under Martian conditions and monitor the process with electrical conductivity measurements. We used the Engineering Qualification Model (EQM) of Habitability: Brines, Irradiation, and Temperature (HABIT)/ExoMars 2022 instrument for the experiment setup but we provide a brief account of constructing a simple and inexpensive electrical conductivity measurement setup. The protocol serves to calibrate the electrical conductivity measurements of the salt deliquescence into brine in a simulated Martian environment. The Martian conditions of temperature (-70 °C to 20 °C), relative humidity (0% to 100%) and pressure (7 - 8 mbar) with carbon-dioxide atmosphere were simulated in the SpaceQ Mars simulation chamber, a facility at the Luleå University of Technology, Sweden. The hydrate form of the known amount of salt accommodated between a pair of electrodes and thus the electrical conductivity measured depends predominantly on its water content and the temperature and relative humidity of the system. Electrical conductivity measurements were carried out at 1 Hz while exposing salts to a continuously increasing relative humidity (to force transitioning through various hydrates) at different Martian temperatures. For demonstration, a day-night cycle at Oxia Planum, Mars (the landing site of ExoMars 2022 mission) was recreated.
Acceso Abierto
Metabolt: An In-Situ Instrument to Characterize the Metabolic Activity of Microbial Soil Ecosystems Using Electrochemical and Gaseous Signatures
(Multidisciplinary Digital Publishing Institute (MDPI), 2020-08-11) Nazarious, Miracle Israel; Zorzano, María Paz; Martín Torres, Javier; Agencia Estatal de Investigación (AEI); Zorzano, M. P. [0000-0002-4492-9650]; Martín Torres, J. [0000-0001-6479-2236]; Nazarious, M. I. [0000-0002-7148-8803]; Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
Metabolt is a portable soil incubator to characterize the metabolic activity of microbial ecosystems in soils. It measures the electrical conductivity, the redox potential, and the concentration of certain metabolism-related gases in the headspace just above a given sample of regolith. In its current design, the overall weight of Metabolt, including the soils (250 g), is 1.9 kg with a maximum power consumption of 1.5 W. Metabolt has been designed to monitor the activity of the soil microbiome for Earth and space applications. In particular, it can be used to monitor the health of soils, the atmospheric-regolith fixation, and release of gaseous species such as N2, H2O, CO2, O2, N2O, NH3, etc., that affect the Earth climate and atmospheric chemistry. It may be used to detect and monitor life signatures in soils, treated or untreated, as well as in controlled environments like greenhouse facilities in space, laboratory research environments like anaerobic chambers, or simulating facilities with different atmospheres and pressures. To illustrate its operation, we tested the instrument with sub-arctic soil samples at Earth environmental conditions under three different conditions: (i) no treatment (unperturbed); (ii) sterilized soil: after heating at 125 °C for 35.4 h (thermal stress); (iii) stressed soil: after adding 25% CaCl2 brine (osmotic stress); with and without addition of 0.5% glucose solution (for control). All the samples showed some distinguishable metabolic response, however there was a time delay on its appearance which depends on the treatment applied to the samples: 80 h for thermal stress without glucose, 59 h with glucose; 36 h for osmotic stress with glucose and no significant reactivation in the pure water case. This instrument shows that, over time, there is a clear observable footprint of the electrochemical signatures in the redox profile which is complementary to the gaseous footprint of the metabolic activity through respiration.
Acceso Abierto
Pressure Optimized PowEred Respirator (PROPER): A miniaturized wearable cleanroom and biosafety system for aerially transmitted viral infections such as COVID-19
(Elsevier BV, 2020-10-06) Nazarious, Miracle Israel; Mathanlal, Thasshwin; Zorzano, María Paz; Martín Torres, Javier; Agencia Estatal de Investigación (AEI); Nazarious, M. I. [0000-0002-7148-8803]; Mathanlal, T. [0000-0003-2691-3855]; Zorzano, M. P. [0000-0002-4492-9650]; Martín Torres, J. [0000-0001-6479-2236]; Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
The supply of Personal Protective Equipment (PPE) in hospitals to keep the Health Care Professionals (HCP) safe taking care of patients may be limited, especially during the outbreak of a new disease. In particular, the face and body protective equipment is critical to prevent the wearer from exposure to pathogenic biological airborne particulates. This situation has been now observed worldwide during the onset of the COVID-19 pandemic. As concern over shortages of PPE at hospitals grows, we share with the public and makers’ community the Pressure Optimized PowEred Respirator (PROPER) equipment, made out of COTS components. It is functionally equivalent to a Powered Air Purifying Respirator (PAPR). PROPER, a hood-based system which uses open source and easily accessible components is low-cost, relatively passive in terms of energy consumption and mechanisms, and easy and fast to 3D print, build and assemble. We have adapted our experience on building clean room environments and qualifying the bioburden of space instruments to this solution, which is in essence a miniaturized, personal, wearable cleanroom. PROPER would be able to offer better protection than an N95 respirator mask, mainly because it is insensitive to seal fit and it shields the eyes as well. The PROPER SMS fabric is designed for single-use and not intended for reuse, as they may start to tear and fail but the rest of the parts can be disinfected and reused. We provide a set of guidelines to build a low-cost 3D printed solution for an effective PAPR system and describe the procedures to validate it to comply with the biosafety level 3 requirements. We have validated the prototype of PROPER unit for air flow, ISO class cleanliness level, oxygen and carbon-dioxide gas concentrations during exhalation, and present here these results for illustration. We demonstrate that the area inside the hood is more than 200 times cleaner than the external ambient without the operator and more than 175 times with the operator and in an aerosol exposed environment. We also include the procedure to clean and disinfect the equipment for reuse. PROPER may be a useful addition to provide protection to HCPs against the SARS-CoV-2 virus or other potential future viral diseases that are transmitted aerially.
Acceso Abierto
Space Environmental Chamber for Planetary Studies
(Multidisciplinary Digital Publishing Institute, 2020-07-18) Ramachandran, Abhilash Vakkada; Nazarious, Miracle Israel; Mathanlal, Thasshwin; Zorzano, María Paz; Martín Torres, Javier; 0000-0003-0499-6370; 0000-0002-7148-8803; 0000-0002-4492-9650; 0000-0001-6479-2236; Unidad de Excelencia Científica María de Maeztu Centro de Astrobiología del Instituto Nacional de Técnica Aeroespacial y CSIC, MDM-2017-0737
We describe a versatile simulation chamber that operates under representative space conditions (pressures from < 10-5 mbar to ambient and temperatures from 163 to 423 K), the SpaceQ chamber. This chamber allows to test instrumentation, procedures, and materials and evaluate their performance when exposed to outgassing, thermal vacuum, low temperatures, baking, dry heat microbial reduction (DHMR) sterilization protocols, and water. The SpaceQ is a cubical stainless-steel chamber of 27,000 cm3 with a door of aluminum. The chamber has a table which can be cooled using liquid nitrogen. The chamber walls can be heated (for outgassing, thermal vacuum, or dry heat applications) using an outer jacket. The chamber walls include two viewports and 12 utility ports (KF, CF, and Swagelok connectors). It has sensors for temperature, relative humidity, and pressure, a UV-VIS-NIR spectrometer, a UV irradiation lamp that operates within the chamber as well as a stainless-steel syringe for water vapor injection, and USB, DB-25 ports to read the data from the instruments while being tested inside. This facility has been specifically designed for investigating the effect of water on the Martian surface. The core novelties of this chamber are: (1) its ability to simulate the Martian near-surface water cycle by injecting water multiple times into the chamber through a syringe which allows to control and monitor precisely the initial relative humidity inside with a sensor that can operate from vacuum to Martian pressures and (2) the availability of a high-intensity UV lamp, operating from vacuum to Martian pressures, within the chamber, which can be used to test material curation, the role of the production of atmospheric radicals, and the degradation of certain products like polymers and organics. For illustration, here we present some applications of the SpaceQ chamber at simulated Martian conditions with and without atmospheric water to (i) calibrate the ground temperature sensor of the Engineering Qualification Model of HABIT (HabitAbility: Brines, Irradiation and Temperature) instrument, which is a part of ExoMars 2022 mission. These tests demonstrate that the overall accuracy of the temperature retrieval at a temperature between -50 and 10 °C is within 1.3 °C and (ii) investigate the curation of composite materials of Martian soil simulant and binders, with added water, under Martian surface conditions under dry and humid conditions. Our studies have demonstrated that the regolith, when mixed with super absorbent polymer (SAP), water, and binders exposed to Martian conditions, can form a solid block and retain more than 80% of the added water, which may be of interest to screen radiation while maintaining a low weight.