© 2020 by the author. Licensee MDPI, Basel, Switzerland.Sobrado, J. M.2024-02-132024-02-132020-10-29Sensors 20(21): 6150(2020)https://www.mdpi.com/1424-8220/20/21/6150#B7-sensors-20-06150http://hdl.handle.net/20.500.12666/940Centro de Astrobiología (INTA-CSIC), Torrejón de Ardoz, 28850 Madrid, Spain. Sobrado, J. M. [0000-0002-7359-0262]. Supplementary materials are available online at https://www.mdpi.com/1424-8220/20/21/6150/s1, Video S1: Dewsensor. Video taken during the injection pulse calibration.Liquid water is well known as the life ingredient as a solvent. However, so far, it has only been found in liquid state on this planetary surface. The aim of this experiment and technological development was to test if a moss sample is capable of surviving in Martian conditions. We built a system that simulates the environmental conditions of the red planet including its hydrological cycle. This laboratory facility enables us to control the water cycle in its three phases through temperature, relative humidity, hydration, and pressure with a system that injects water droplets into a vacuum chamber. We successfully simulated the daytime and nighttime of Mars by recreating water condensation and created a layer of superficial ice that protects the sample against external radiation and minimizes the loss of humidity due to evaporation to maintain a moss sample in survival conditions in this extreme environment. We performed the simulations with the design and development of different tools that recreate Martian weather in the MARTE simulation chamber.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttps://creativecommons.org/licenses/by-nc-nd/4.0/Mars simulationArtificial atmosphereWater cycleMoss survivalMimicking the Martian Hydrological Cycle: A Set-Up to Introduce Liquid Water in Vacuuminfo:eu-repo/semantics/article10.3390/s202161501424-8220info:eu-repo/semantics/openAccess